Thermo Scientific Nunc surfaces

Surfaces for ELISA
The choice of the optimal surface for the binding or adsorption of the fi rst layer in a sandwich ELISA is critical for the success of the procedure. While high binding is clearly important, the most important parameter is the detection limit which depends on the signal to noise ratio. Use the Nunc triangle (for passive adsorption surfaces) or the hexagon (for active binding) as a guide when selecting the surface for a new assay. To achieve the best possible results always optimize the incubation conditions and concentrations of the assay components.

MaxiSorp™
Nunc MaxiSorp is treated to give a mixture of hydrophobic and hydrophilic areas for the adsorption of proteins. It is particularly optimized for the adsorption of IgG in a sandwich ELISA.

MediSorp™
When high ELISA background readings are a problem, MediSorp may be the solution. MediSorp’s lower total adsorption capacity can, in many cases, reduce non-specifi c adsorption of serum proteins giving an improved signal to noise ratio.

PolySorp™
The PolySorp surface is ideal for the adsorption of hydrophobic molecules, including those that contain lipid moieties.

MultiSorp™
This highly hydrophilic surface has a number of applications. Use MultiSorp for the adsorption of hydrophilic biomolecules such as highly substituted glycoproteins. Alternatively this surface could be
used for the assay, in liquid phase of very hydrophobic analytes.

Streptavidin
The interaction between biotin and streptavidin has been exploited in many assay applications. Nunc provides two types of streptavidin surface.

Streptavidin Passive Attachment
The streptavidin molecule can be adsorbed directly to the polystyrene surface. For many applications this works well, particularly for relatively small biotinylated molecules where steric hindrance due to closeness to the polystyrene presents no problem.

Immobilizer™ Streptavidin
In this case the streptavidin molecules are covalently bound to the surface via spacer approx. 2 nm in length. Th is produces a number of advantages: reduced steric hindrance, a very stable surface and controlled spacing of the available binding sites, which is ideal for large biotinylated biomolecules.

Immobilizer Amino
In an ELISA, there are many advantages of covalently binding the first layer to the surface. The Amino surface can be used to covalently bind proteins (including antibodies) or peptides. With a modifi ed protocol DNA can also be bound. Once the fi rst layer is bound it is extremely stable allowing rigorous washing in the subsequent steps.

Immobilizer Nickel-Chelate/ Immobilizer Glutathione
For determination and characterization of fusion proteins, Nunc™ Brand off ers two possibilities. Nickel-Chelate for Histidine-tagged and Glutathione for GST-tagged fusion proteins. These plates can be used for purified samples, as well as crude cell lysates. Both Immobilizer Nickel-Chelate and Glutathione have all the advantages of the Immobilizer family of products: no activation steps, low detection limit, high signal to noise ratio and low background without blocking.

CovaLink™
The CovaLink surface chemistry often allows binding of molecules, where other methods have failed. It can be used with bifunctional linkers to bind peptides via a carboxylic acid group or DNA by the phosphorylated 5’ end of the molecule DNA. Alternatively, the whole surface can be activated using glutaraldehyde.

Thermo Scientific Surfaces

Immulon® 1 B
Consistent medium binding for hydrophobic complexes and proteins

Immulon 2 B
High binding surface to provide increased binding of hydrophilic proteins and complexes

Immulon 4 HBX
Extra-high binding surface with special resin - offers maximum protein uptake
Well-to-well CV’s of plates is certified

Microfluor 1 White
Medium binding - offers high signal reflectance and reduced background fluorescence

Microfluor 2 White
Microplate for assays that require high binding and reduced background for fluorescence assays in the ultraviolet range

Microfluor 1 Black
Fluorescence vessel with low background

Microfluor 2 Black
Irradiated Microtiter microplate that provides higher binding and minimal background fluorescence

Microlite 1+
Ideal for low signal luminescent reactions, offering medium binding with enhanced dynamic range, extra high reflectivity and minimal cross talk

Microlite 2+
High binding surface with enhanced dynamic range, extra high reflectivity and minimal cross talk for low signal luminescent reactions